JCS -- Chang et al. 119 (6): 1144 Figure IG5

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Fig. 5. incenp^EC3747enlarged CNS cells lack Drosophila Incenp and phosphorylated histone H3. (A-A'") Mitosis in a wild-type CNS cell showing Drosophila Incenp localized to the centromere at prometaphase. Histone H3 is phosphorylated in the mitotic chromosomes. (B-B'") Enlarged cells in the CNS of Incenp^EC3747 embryos do not show any Drosophila Incenp phosphorylated histone H3 staining. In the merged figures, DAPI is shown in blue, phosphorylated histone H3 in red, Drosophila Incenp in green. Enlarged CNS cells in incenp^EC3747 embryos are polyploid and show multiple centrosomes. (C-C'") Wild-type cell with Drosophila Incenp localized to centromeres at metaphase (arrowhead) and in the midzone at telophase (arrow). (D-E'") Enlarged CNS cells are polyploid and show either and abnormal accumulation of ${gamma}$ -tubulin in centrosomes or multiple centrosomes. The two centrosomal masses in D-D'" have not separated properly in this cell. Multiple ${gamma}$ -tubulin spots in E-E'". In the merged figures, DAPI is shown in blue, ${gamma}$ -tubulin in red, Drosophila Incenp in green. Bar, 10 µm.