Fig. 6. Localization of the cell-fate determinant Prospero in mitosis of wild-type and incenp^EC3747 embryos. (A-C) Localization of Prospero in mitosis of wild-type CNS cells. (A) In early prophase, Drosophila Incenp shows general association with chromatin, whereas Prospero localizes in discrete spots associated with chromatin. (B) By early prometaphase, both proteins become closer although they do not colocalise totally. (C) In metaphase, Drosophila Incenp remains associated with centromeres, whereas Prospero is localized in a crescent in the basal cell cortex. (D-D') In incenp^EC3747 homozygous embryos, we observed enlarged polyploidy cells depleted of Drosophila Incenp in which Prospero localization is abnormal. All images are projections of series of deconvolved images. Bars, 5 µm.