Fig. 4. MidA is a mitochondrial protein. (A) Scheme of the construct that was used for the expression of MidA fused to GFP. Coding sequences are depicted as open boxes and the intron by a thin line. The expression of the fused genes was driven by the actin 15 promoter. (B) Colony size after transfection of the construct A15::midA-GFP into midA^- cells. The indicated strains were mixed with Klebsiella aerogenes and plated on SM plates. Photographs were taken after 5 days at 22°C. Bar, 1 cm. (C) Cells expressing midA fused to GFP were incubated with the mitochondrial marker MitoTracker Red, fixed and observed in a confocal microscope. Series of 2-µm confocal images from the same cell show GPF location in green and the mitochondrial marker in red. Colocalization can be seen in the merged images. Bar, 10 µm.