Fig. 7. Random migration of cells expressing HA1, SW1 or SßX on collagen IV or fibronectin. Cells were sparsely plated onto glass-bottomed culture dishes coated with fibronectin (FN) or collagen IV (CN) and allowed to spread for 1 hour. Dishes were then placed on a heated microscope stage and images captured every 20 minutes for 10-12 hours. Data are average velocity measured by tracking cell nuclei of at least 10 cells from each of two (HA1 and SW1) or three (SßX) experiments (±s.e.m.). Bars with different shadings are significantly different from each other (Student's t test, P<0.01).