(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.


Figure 6


Fig. 6. Follicle cell microvilli are abnormal in Cad99C mutants. (A-C) Follicle cells of stage-10 (A) heterozygous Cad99C51C/+, (B) mutant Cad99C57A/51C, or (C) mutant Cad99C57A/120B egg chambers expressing CD8-GFP (CY2, UAS-CD8-GFP) stained for GFP. Cad99C mutant flies have abnormal CD8-GFP-labeled protrusions compared with control flies. Apical is to the top. (D-F) Clones of homozygous mutant Cad99C57A/57A follicle cells of stage-10 egg chambers stained with Rhodamine-Phalloidin marked by the absence of Cad99C. Cad99C staining is shown in (D) and Rhodamine-Phalloidin staining in (F). (E) Merge of (D) and (F). Phalloidin staining detects microvilli in between control follicle cells and the oocyte (arrowhead), but not between mutant follicle cells and the oocyte (arrow). Note that the distance between the oocyte and the Cad99C57A/57A follicle cells is increased. Oo, oocyte; Fc, Follicle cells. Bars, 5 µm (A-D).