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Figure 8


Fig. 8. The extracellular region fused to the transmembrane domain of Cad99C is sufficient to promote the formation of large bundles of follicle cell microvilli. (A) Scheme of Cad99C-HA, Cad99C-EXTRA-HA and Cad99C-INTRA-HA. Cad99C-EXTRA-HA lacks the entire cytoplasmic region, whereas Cad99C-INTRA-HA lacks most of the extracellular region. Full-length and deletion mutants of Cad99C all have a triple HA tag at their C-terminus. Sp, signal peptide; TM, transmembrane domain; PDZ-BS, putative PDZ domain-binding site. (B-E) Electron micrographs of anti-HA 12 nm immunogold-labeled sections of stage-10 (B) control egg chambers (Act5C>GAL4) and egg chambers expressing (C) Cad99C-HA (Act5C>GAL4, UAS-Cad99C-HA), (D) Cad99C-INTRA-HA (Act5C>GAL4, UAS-Cad99C-INTRA-HA), and (E) Cad99C-EXTRA-HA (Act5C>GAL4, UAS-Cad99C-EXTRA-HA) in clones of follicle cells. (C-E) Insets show higher magnification views. Overexpression of Cad99C-HA or Cad99C-EXTRA-HA results in the formation of large HA-labeled bundles of follicle cell microvilli. Oo, oocyte; Fc, follicle cells. Bars, 500 nm (B-E).