Fig. 10. Suppression of hVps34 expression potentiates EGFR signaling. To measure EGFR phosphorylation and signaling, the control and KD cells were harvested at the indicated times after the addition of EGF and subjected to immunoblot analysis for (A) phospho-EGFR (pEGFR) and total EGFR, or (B) phospho-ERK1/2 (pERK) and total ERK1/2. The bar graphs illustrate the data generated from Kodak Imager scans of blots from triplicate cultures of each cell line.