Fig. 3. Vacuoles in the hVps34 KD cells are membrane-bound structures with occasional internal vesicles and electron-dense material. Control and hVps34 KD cells were examined by electron microscopy. (A) Control and KD cells magnified 3,900x. Bar, 5 µm. (B) Highlighted region of KD cell from panel A at 21,000x. Bar, 1 µm. Labeled structures: m, mitochondria; n, nucleus; v, vacuole. The arrowhead points to a single membrane surrounding the vacuole. Arrows point to occasional small internal vesicles and electron-dense material.