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Figure 4


Fig. 4. Effect of the adipogenic cocktail on the transcription levels of leptin, IL-6, C/EBPß and C/EBP{delta} genes analyzed by RT-PCR. Cells were incubated for the indicated times with the adipogenic cocktail before RNA extraction, reverse-transcription and amplification in the presence of SYBR Green and specific primers. TBP was used as a house-keeping gene for data normalization.