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Figure 3


Fig. 3. Mimicking Wnt signalling in osteoblasts stimulates ß-catenin nuclear translocation and TCF/LEF-dependent transcriptional activation. (A) Inhibition of GSK3ß with LiCl (20 mM) stimulated ß-catenin nuclear translocation in SaOS-2 cells (arrows) compared with NaCl controls. Bar, 5 µm. (B) Inhibition of GSK3ß with LiCl (20 mM) stimulated TOPFLASH TCF/LEF gene transcription in SaOS-2 cells compared with cells incubated with vehicle alone or those transfected with control reporter containing mutant TCF/LEF binding sites (FOPFLASH). Mean values are shown ± s.d., n=4. (C) Time course of LiCl-stimulated gene transcription in MG-63 cells. LiCl stimulated TCF/LEF reporter gene transcription from 5 hours post treatment (mean ± s.e.m., n=8; ***P≤0.001).