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Figure 4


Fig. 4. ß-catenin transactivates TCF/LEF gene transcription in osteoblasts. (A) Overexpression of ß-catenin EGFP fusion protein significantly increased TCF/LEF-dependent transcription of a luciferase reporter gene (TOPFLASH) in MG-63 cells compared with cells transfected with control reporter containing mutant TCF/LEF binding sites (FOPFLASH). By contrast, overexpression of a ß-catenin C-terminal mutant lacking the transactivation domain (ß-catenin {Delta}C695-781) failed to activate transcription (values are mean ± s.d., n=4; ***P≤0.001; n.s., not significant). (B) Both full-length ß-catenin and ß-catenin {Delta}C localised to the nucleus of osteoblasts (arrows). EGFP, Green; DAPI, blue. Bar, 10 µm.