Fig. 7. Expression of LRP5 and LRP6 Wnt co-receptors by osteoblasts, osteoclast progenitors and mature osteoclasts. (A) RT-PCR demonstrating expression of LRP5 and LRP6 using cDNA generated from human osteoblastic cells (MG-63, SaOS-2 and TE85), primary human osteoblasts (HOB), mouse spleen and mature osteoclasts derived from human long-term bone marrow cultures. (B) Histogram illustrating the purity of CD14⁺ cells determined by flow cytometry following isolation from the mononuclear (MNC) fraction of human peripheral blood by magnetic activated cell sorting. RT-PCR demonstrating expression of LRP5 and LRP6 by CD14^- (depleted) and CD14⁺ (enriched) cells is shown on the right. The PCR product obtained using SaOS-2 cDNA is shown for comparison.