Fig. 9. Evidence for endogenous Wnt signalling in mature osteoclasts. Fluorescent immunolocalisation of ß-catenin in mature disaggregated rat osteoclasts. ß-catenin predominately localised to nuclei with some peripheral staining (green). Multinucleation is disclosed by DAPI (blue) and peripheral actin ring formation, typical of active bone resorbing osteoclasts is stained with Rhodamine-phalloidin (red). Bar, 10 µm.