Fig. 7. Displacement mapping analysis of cell and fibril movement (see Materials and Methods for description of technique). Frequency distribution plots showing displacements of point markers positioned on cell, LTBP1 or fibronectin fibril images during a time interval of 1 hour in day 2 (A) and day 12 (B) FRC cultures. Note that the movement of cells, LTBP1 and fibronectin (FN) fibrils in day 2 cultures are very similar. However, in day 12 cultures, the number of markers that moved distances greater than 6 µm was higher for fibronectin compared with LTBP1. Mean displacements for fibronectin and LTBP1 markers were significantly different at day 12 but not day 2 (see text).