Fig. 9. Post-translational modifications of Orc1 affected the subcellular localization of Orc1 and suppressed changes in cell morphology characteristic of apoptosis. HeLa cells were transfected with 1 µg pFHsOrc1-Ub (Orc1-Ub). CHO cells were transfected with 1 µg of either pFCgOrc1(A5) or pFCgOrc1(D5). At 12 and 24 hours post-transfection, cells were stained with anti-FLAG antibody to detect FCgOrc1 (red) and with Hoechst to detect nuclear DNA (blue). Merged images revealed FCgOrc1 localization relative to DNA, and DIC images revealed the morphology of the cell. DIC, differential interference contrast.