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Figure 1


Fig. 1. Trafficking of full-length and truncated PC2 to cilia. (A) Expression of full-length PC2 with a COOH-terminal EGFP tag in the cilia of LLC-PK1 (top panel) and MDCK (bottom panel) cells. (B) Expression of PC2-L703X with a COOH-terminal EGFP in the cilia of LLC-PK1 (top panel) and MDCK (bottom panel) cells. Cells expressing PC2-L703X form longer cilia more readily than cells expressing only native PC2 or the full-length PC2 construct. (Left panels) EGFP epifluorescence (green) shows expression of PC2, (middle panels) acetylated {alpha}-tubulin (red) marks the cilia; (right panels) merged images. Bars, 10 µm.