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Figure 2


Fig. 2. PC2 traffics to cilia independently of PC1. (A) PC2 is expressed in cilia at the embryonic node in Pkd1-/- mice. (B) PC2 is expressed in cilia (arrow) of cells lining kidney cysts formed in Pkd1 mutant mice. (C) PC2 is expressed in the cilia (arrows) in cultured mouse kidney cells lacking PC1. (D) PC2-GFP fusion protein expressed in cells lacking PC1 traffics into cilia as evidenced by EFFP epifluorescence in cilia. Adjacent cells not expressing the transfected protein do not show EGFP epifluorescence. (A-C) Anti-PC2 (YCC2), green; (D) EGFP epifluorescence, green; (A-D) anti-acetylated {alpha}-tubulin, red; right panels, merged images. Bars, A, C, D, 10 µm; B, 5 µm. Insets show selected cilia in each field.