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Figure 3


Fig. 3. Amino acids 5-72 of PC2 are necessary for its trafficking to cilia. Deletion of amino acids 5-72 in either (A) the PC2-L703X truncated peptide [({Delta}5-72)PC2-L703X] or (D) the full-length PC2 [({Delta}5-72)PC2] with an intact COOH terminus results in PC2 absence in the cilia. Robust expression of the respective proteins at the level of the cell body is shown in the right-most panel as well as in the xz-plane reconstructions in these confocal images. (B,C) Deletion of amino acids (B) 72-130 [({Delta}72-130)PC2-L703X] or (C) 130-220 [({Delta}130-220)PC2-L703X] does not abrogate trafficking of the respective peptides into cilia. Anti-HA epitope (green) indirect immunofluorescence indicates expression of PC2 constructs and acetylated {alpha}-tubulin (red) marks the cilia; right panels, merged images. The cell lines were made in LLC-PK1. Bars, 10 µm.