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Figure 6


Fig. 6. The N-terminal domain of PC2 can direct cilial localization of heterologous non-cilial proteins. (A) Addition of the first 72 amino acids of PC2 to PKD2L1 results in trafficking of the chimeric protein, (1-72-PC2)-PKD2L1, into cilia (arrows). (B) Replacing the N-terminal region of PC2-L703X with the N-terminal region of PKD2L1 results in failure of the chimeric protein, PKD2L1-{Delta}(1-328)PC2-L703X, to traffic to cilia. (C) A doubly chimeric protein in which the first 72 amino acids of PC2 are fused to the PKD2L1-{Delta}(1-328)PC2-L703X chimera localizes to cilia. Anti-HA, green; anti-acetylated {alpha}-tubulin, red; right panels, merged images. Bars, 10 µm.