Fig. 9. RVxP is necessary for cilial targeting of PC2. (A) Alignment of the first 31 amino acids of PC2 from several species. ^*, location of mutations in the most conserved residues whose cilial trafficking properties are shown in B-E. +, other mutation sites tested that had no effect on cilial localization. Identical residues are highlighted and boxed; residues in red abrogated cilial location of PC2-L703X. (B) Amino acid substitution S4Adoes not affect trafficking of PC2-L703X to cilia. (C-E) Substitutions R6G (C) V7A (D) and P9A (E) result in loss of cilial localization of PC2-L703X. Green, anti-HA; red, anti-acetylated -tubulin, third row, merged images; far right panels in C-E are confocal sections at the level of the nucleus showing robust expression of the respective mutant proteins in all cells. Bars, 10 µm.