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Figure 4


Fig. 4. Connections of conduits to blood vessels and Hassal's bodies. (A-C) Double immunofluorescence staining of a thymic cryostat section with antibody against the laminin {gamma}2 chain (A) and the antiserum against fibrillin-1 (B) revealed a colocalization of both antigens in the conduits; by contrast, only fibrillin-1 can be detected in the blood vessels. In the merged picture (C), a nuclear DAPI staining is included. (D-F) Conduits are also connected to Hassal's bodies as shown in the double immunofluorescence staining using antibody against the laminin {gamma}2 chain (D) and an antiserum against ß-catenin (E). In the overlay picture (F), which also includes the nuclear blue staining, the direct association of the conduits with the Hassal's body can be observed. Bar, 10 µm.