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Figure 3


Fig. 3. Inactivation of SEC14 blocks vesicle accumulation at the SPB in spo14 mutants. sec14-1/sec14-1 (Y5671) and sec14-1/sec14-1 spo14 {Delta}/spo14 {Delta} (Y5688) cells carry pRS424-Dtr1p-GFP were sporulated at either the permissive temperature (24°C) or the nonpermissive temperature (35°C) and examined by fluorescence microscopy. SPBs were visualized using an RFP fusion to Mpc54p. Bars, 5 {Delta}m.