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Figure 6


Fig. 6. Prospore membrane proteins accumulate at the SPB in sec1 and spo20 sec9 but not mpc54 mutants. sec1ts/sec1ts (AN127), sec9ts/sec9ts, spo20 {Delta}/spo20 {Delta} (AN211) mpc54 {Delta}/mpc54 {Delta} (NY541) cells expressing GFP-Spo20p51-91 (from pRS426-G20 or pRS424-G20) were sporulated and examined by fluorescence microscopy. DNA was visualized using DAPI. Bars, 1 {Delta}m.