Fig. 5. Effect of KGF treatment on 2ß1^hi cells. (A) Bar chart summarising the effect of KGF on expression of differentiation markers PAP, CK18 and AR (measured by FACS) in 2ß1^hi cells. (B) Triple immunofluorescence study of 2ß1^hi cells treated with KGF. AR, TRITC/red; PSA, FITC/green; DAPI, blue. Colocalisation is indicated in yellow. (C) RT-PCR for KGFR mRNA expression in primary prostate cell sub-populations. ^*PEC, primary culture of epithelial cells following subtraction of ₂ß₁^hi basal cells. ß-actin levels were detected as a control (lower gel). (D) Summary of FACS analysis of AR expression in 2ß1^hi cells untreated or treated with combinations of KGF and the ß1-blocking antibody. Data are mean ± s.e.m. of three experiments.