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Figure 6


Fig. 6. Immunofluorescence analysis of keratins and phospho-keratins under isosmotic and hyposmotic conditions. HT29 (A) and Caco2 (B) cells were cultured in isosmotic (Iso) or hyposmotic (Hypo) conditions (6 hours, 37°C), then fixed and triple (K8/K18, K8 Ser431-P and nuclei in HT29 cells) or double (K8/K18, K8 Ser431-P in Caco2 cells) stained. The panels on the right are enlargements of the outlined `boxed' areas. Bars, 25 µm.