Fig. 7. EMSA analysis of OLIG2 binding to an oligonucleotide containing the CTF site of the p27^Kip1 gene promoter. (A) Immunoblot analysis of nuclear extracts prepared from U12-1 cells cultured for 48 hours in Tet-on or Tet-off medium. The same membrane was probed with antibodies to OLIG2, to p27^Kip1, and to lamin A/C (control). (B) EMSA was performed with a ³²P-labeled oligonucleotide corresponding to positions -533 to -513 of the human p27^Kip1 gene promoter. The corresponding unlabeled oligonucleotide was used as a competitor. The arrowhead indicates a specific DNA-protein complex, and the arrow indicates unbound probe.