Fig. 7. nELAV-dependent upregulation of Msi1 protein after phorbol ester treatment. (A) After stimulation for 15 minutes with 100 nM PMA or DMSO, cell fractions (cytosol, membranes and cytoskeleton) were separated from human SH-SY5Y cells. Samples were analysed by western blotting with the indicated antibodies. (B) Densitometric analyses of western blot data normalized to -tubulin protein content (mean ± s.d.; n=3, ^*P<0.05, ^***P<0.001, Student's t-test).