Fig. 5. Axin and axin2 slow down ß-catenin in the cytoplasm and do not accelerate ß-catenin nucleo-cytoplasmic shuttling. (A) Live cell images of mYFP-ß-cateninS45A (upper panels) and co-expressed mCFP-tagged axin, axin2 or axinC (lower panels). Bar, 10 µm. (B) Compartment bleach analysis of mYFP-ß-catenin in the absence or presence of axin, axin2 or axinC. (C) Cytoplasmic point bleach analysis of mYFP-ß-catenin in the absence or presence of axin, axin2 or axinC. The mobilities of mYFP-tagged axin, axin2 and axinC were determined in separate experiments. The cartoons in B and C designate compartment bleaches and point bleaches, respectively.