Fig. 4. Genetic divergence of clonal populations was of the same order in two successive cloning processes. The index of genetic divergence was assessed by AP-PCR (see Materials and Methods). The X-axis represents the divergence between each expanded cell population cloned directly from the parental cells (Cloning 1) versus (vs) the corresponding parental cells. The Y-axis represents the divergence between each of the second-generation clones (Cloning 2, expanded from a clone from the first cloning) versus the clone from which they were isolated. The diagonal represents ratio 1, corresponding to equal levels of genetic divergence in both cloning processes.