Fig. 1. Co-sedimentation of dynamin II and syndapin II. Cytosolic proteins from HepG2 cells were centrifuged through a 10-30% glycerol gradient and fractions were analyzed by immunoblotting with the anti-dynamin II antibody C12 (A) and the anti-syndapin II antibody 3685 (B). The corresponding molecular masses were determined by positions of the marker protein catalase (230 kDa), aldolase (160 kDa) and bovine serum albumin (67 kDa), run on parallel gradients.