Fig. 3. Reversible redistribution of II spectrin is a downstream event of M1 mAChR activation. Redistribution of YFP-II spectrin starts approximately 42 seconds after Oxo-M application and lasts for 370 seconds in M1-CHO cells. II spectrin redistributes into the bleb-ends (arrows) during blebbing and reverts to the pre-activation positions (arrowheads) when stabilized. For the live image of this cell, see supplementary material Movie 1. Bar, 10 µm.