Fig. 7. Expression of nNOS is not restored by expression of Dp116. (A) Immunofluorescence staining of quadriceps muscles with a polyclonal antibody against nNOS. Dp116/mdx4cv and transgenic/mdx muscles expressing Dp260,
H2-R19 (mini-dystrophin), and
R4-23 (micro-dystrophin) are shown. Bar, 100 µm. (B) Western analysis of nNOS from pellet (microsomal) and supernatant (soluble) fractions of KCl-washed microsome preparations from C57Bl/6 (1), mdx4cv (2), Dp116/mdx4cv (3), and
R4-23/mdx (4) skeletal muscles. An identical gel stained in parallel with Coomassie Blue is shown as a loading control.