Fig. 3. Twf1 and Cof1 promote actin filament disassembly in dilution-induced assays. (A,B) Time course of unlabeled actin filament light scattering (monitored at 400 nm) after dilution at time zero, from 5 µM to 0.25 µM, in the presence of indicated proteins at pH 5.0 (A) or pH 7.5 (B). A.U., arbitrary units. (C) Fluorescence micrographs of actin filaments incubated alone or with 100 nM Cof1 or Twf1 as in Fig. 1B. At the indicated time points, samples were removed from dilution-induced reactions, incubated with Alexa Fluor 488-congugated phalloidin, and visualized by light microscopy. Bar, 5 µm.