Fig. 8. The subcellular distribution of the palmitoylated proteins PSD-95, GAP-43 and NOS2 is different in COS7 cells, C2C12 myotubes and H9C2 cardiac myocytes. (A) Both PSD-95-GFP and GAP-43-GFP constructs were transfected in COS7 cells and the subcellular distribution was analysed with the Golgi marker ß-cop labelled with a Cy3 secondary antibody (red). The right panels show the merges of the three fluorescence signals. Cell nuclei were stained with Hoechst 33258 (blue). (B) Mouse C2C12 myoblasts were converted into myotubes and a PSD-95 construct was transfected in the presence of a mixture of the cytokines LPS and IFN- that induce NOS2 expression. Inducible NOS is present to differing degrees in most of the cells with a patchy distribution (red staining). A magnified image of the boxed area in the merged image shows the colocalisation more clearly (right panel). (C) H9C2 rat cardiac myocytes were transfected with a PSD-95 construct in the presence of a mixture of the cytokines LPS and IFN that induce NOS2 expression. Bars, 50 µm.