Fig. 2. Proportion of muscle fibers showing predominantly nuclear localization of NFATc1-GFP in normal mouse soleus and TA muscles, and in muscles subjected to various experimental conditions. Values are expressed as mean ± s.e.m. Muscles were transfected with plasmid coding for NFATc1-GFP and 7 days later were either immediately removed for analysis (control) or subjected to various experimental conditions: anesth, 2 hours anaesthesia; den, 2 hours denervation; overload, soleus muscle from contralateral leg; cain, soleus muscle co-transfected with the calcineurin inhibitor cain; 100 Hz or 20 Hz, TA muscles stimulated for 2 hours at 100 Hz or 20 Hz, respectively; 20 Hz + cain, TA muscles co-transfected with cain and stimulated for 2 hours at 20 Hz.