Fig. 6. CNT2-mediated adenosine transport is required for AMPK activation in IEC-6 cells. Cells were treated with 1 µM adenosine (Ado), either in the absence or presence of 100 µM formycin B (FB) for 10 minutes, and then processed as indicated previously. (A) 1AMPK activity (mean ± s.e.m.) from four independent experiments. (B) A representative western blot of phosphorylated ACC (P-ACC) and corresponding densitometric analysis of the gels (mean ± s.e.m.). (C) Cells were incubated with 1 µM adenosine, either in the absence or the presence of 1 µM NBTI and 1 µM dipyridamole (Dip) for 10 minutes. A representative western blot of phosphorylated ACC and the corresponding densitometric analysis (mean ± s.e.m.) is shown. The statistical significance was assessed by Student's t-test: A: *P<0.05, **P<0.01; B: *P<0.05, ***P<0.001; C: **P<0.01. Ctrl or C, control.