Fig. 8. Adenosine phosphorylation is necessary for AMPK and ACC phosphorylation in FAO cells. The effect of adenosine kinase inhibition on the increase in AMPK and ACC phosphorylation triggered by adenosine (Ado) was monitored after pre-incubating the cells with 0.2 µM 5'-iodotubercidin (ITU) for 30 minutes. Two representative western blots of phosphorylated AMPK (P-AMPK) (A) and phosphorylated ACC (P-ACC) (B), and corresponding densitometric analysis (means ± s.e.m.) of six independent experiments are shown. Statistical significance was assessed by Student's t-test: **P<0.01; *P<0.05. Ctrl or C, control.