Fig. 5. NMDA-treatment downregulates AUF1 levels in granule cells. (A) Whole-cell lysate (20 µg) of (1) control or (2) NMDA-treated cells, lamin B1 was immunodetected as loading control. (B) Nuclear or cytosolic extracts (N or C, respectively) from control or NMDA-treated cells. Lamin B1 (a nuclear protein) and GAPDH were immunodetected as loading control.