Fig. 3. Chimeras that contain oligomerized, but not monomeric, cytoplasmic domain of the integrin ß subunit recruited talin head and FAK, but not other integrin-associated proteins. (A) A chimera containing the extracellular domain of monomeric CD2 fused to the cytoplasmic and transmembrane domains from ßPS (cßß), did not increase talinH-GFP recruitment to the muscle ends. (B) The `diß' chimera, containing extracellular and transmembrane domains of the oligomeric and/or dimeric protein Torso, recruited talinH-GFP out of the nucleus to the cortex. (C) Bar graphs show the average ratio of cortical to cytoplasmic GFP staining in living embryos for wild-type muscles and muscles overexpressing integrin and integrin cytoplasmic tail chimeras. Overexpression of integrins or expression of the diß chimera resulted in the recruitment of talinH-GFP to the cell cortex whereas expression of the cßß and ccß chimeras did not. (D) Examination of other proteins showed that the majority were not recruited by diß and the ratio of cortical to cytoplasmic stain was not changed between wild-type muscle and muscles that expressed diß. However, phosphorylated FAK was weakly recruited to the cortex by diß.