Fig. 4. diß interferes with the endogenous integrin-cytoskeleton link and recruits talin. (A-C") The dominant negative phenotype in muscles expressing diß. (A) Integrin (anti-PS2, blue in A and white in A") remained with the tendon matrix (anti-tiggrin, red in A and white in A') rather than at the ends of the detached muscles (myristylated-GFP, (green in A and white in A"'). By contrast, talin was found at the ends of the detached muscles (red in B, arrowheads in B"), distinct from PS2 (green in B and B') and colocalized with diß (C). The later-stage muscles (C) contained intracellular accumulations of talin and diß that were not seen in earlier-stage muscles (B). Arrowhead in A', A"', C indicate ends of detached muscles. Bar, 20 µm.