Fig. 6. Expression levels and localization of mutant forms of diß to muscle ends. (A) Western blot analysis shows relative levels of expression of diß and its mutated variants as expressed under the control of the mef2::Gal4 driver; actin is used as a loading control. The diß protein has 12 potential extracellular glycosylation sites and is similar to the wild-type Torso protein (Sprenger and Nusslein-Volhard, 1992), two bands are detected for diß. (B,C) Antibody staining reveals that the mutant forms of diß still localized to muscle ends (arrowheads) as illustrated for the N840A and D807V/E810A mutations. Bar, 20 µm.