Fig. 7. Recruitment of talin head by diß mutants. (A) diß recruited talinH-GFP from the nucleus to the cell cortex of muscles of living late-stage embryos. (B) Mutating the proximal NPXY motif abolished this cortical recruitment in muscle. (C) A mutated ATST sequence in the integrin cytoplasmic tail still recruited talin head out of the nucleus and onto the cortex. (D) Quantification of the average ratio of cortical to cytoplasmic staining from multiple muscles showed that mutations in the ATST sequence of the integrin cytoplasmic tail or in the distal NPxY motif still recruited talin head to the muscle cell cortex, whereas mutations in the proximal region or the first NPxY did not. (E-E") In the absence of endogenous integrins (E", lacking of PS2) the diß mutant with a TST to AAA mutation in the integrin cytoplasmic tail (E') recruited endogenous talin to the muscle ends (E, arrow). Bar, 20 µm.