Fig. 6. Inhibition of PLD blocks cell motility in corneal epithelial cells. (A) Inhibition of healing in wounds of confluent layers of cells. Healing was allowed to proceed in the presence of EGF (200 ng/ml), and alcohols [1-butanol (1-BuOH) and tert-BuOH] (0.45%). (B) Inhibition of EGF-induced chemokinesis. Cells were seeded in Transwell^® migration chambers in the presence of the indicated compounds in both the top and bottom chambers. Alcohols were used at 0.40%, and C₈-PA at 125 µM. (C) Cells were pre-treated with the indicated concentrations of 1-butanol for 8 hours. The 1-butanol was washed out, and the cells were allowed to migrate for another 8 hours in the presence of EGF.