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Figure 1


Fig. 1. Formation of a centrosomal actin bundle (CAB) in neuronal cells transfected with GFP-espin (green). (A-C) Colocalization of GFP-espin (B) and F-actin labeled with Texas-Red-phalloidin (C, red) in a large perinuclear actin bundle in a transfected PC12 cell (A, phase). (D,E) GFP-espin (E) in a large perinuclear actin bundle in a transfected primary hippocampal neuron (D, phase). (F-H) GFP-espin in bundle assembly intermediates observed in PC12 cells 4-8 hours after transfection (in order of appearance) highlighting relationship to microtubule-organizing center labeled with {gamma}-tubulin antibody (red dots). (I) Inhibition of CAB formation upon cotransfection of PC12 cell with GFP espin and myc-dynamitin constructs. (Dynamitin expression was confirmed with myc antibody; not shown.) (J) Absence of CAB formation in a PC12 cell transfected with GFP-espin WH2-domain-deletion construct. (K) Restoration of CAB formation in a PC12 cell transfected with the GFP-espin WH2-domain-deletion construct containing an upstream WH2 domain. Blue, nuclei labeled with DAPI. Bars, 5 µm.