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Figure 4


Fig. 4. Formation of nuclear actin bundle (NAB) in LLC-PK1 cells transfected with GFP-jerker espin 2B (green). (A-C) Colocalization of GFP-jerker espin (B) and F-actin labeled with Texas-Red-phalloidin (C, red) in a large actin bundle in a transfected cells (A, phase; blue in B, DAPI). (D) Confocal image showing that the GFP-jerker espin-labeled actin bundle is internal to the nuclear lamina revealed by lamin antibody (red). (E-T) Four rows of NAB assembly intermediates observed 4-8 hours after transfection (in order of appearance) highlighting the relationship to the nucleolus (phase dense) at early stages. Bars, 15 µm (B); 5 µm (D,F).