Fig. 7. SH2-Bß promotes neurite outgrowth in cultured mesencephalic neurons. Cultured mesencephalic neurons were co-transfected with pEGFP-N2 in the ratio of 1:10 together with pcDNA3 (control), pcDNA3-myc-SH2-Bß or R555E. Images were taken under fluorescence microscopy (magnification 20x) to identify GFP-positve neurons. (B) Quantification of neurite outgrowth in cultured mesencephalic neurons. Metamorph image-analysis software was used to quantify the length of the longest neurite. Data are presented as the mean ± s.d. of three independent experiments performed in triplicate experiments. **, significant difference between SH2-Bß/GFP and pcDNA3/GFP control (P<0.01, factorial ANOVA); ^##, significant difference between R555E/GFP and control (P<0.01, factorial ANOVA).