Fig. 4. Dynamic incorporation of EGFP-caldesmon into podosomes in live cells treated with PDBu. Video images of live A7r5 cells expressing EGFP-l-caldesmon and DS Red-cortactin were recorded either in the absence (A) or presence (B) of 1 µM PDBu. The times in A refer to the time from the beginning of the video; the times in B refer to the time after the onset of PDBu stimulation. Bars, 20 µm.