Fig. 4. Immunoblot analyses of uterine tissue homogenates of two untreated and tamoxifen-treated Cx43fl/fl:SM-CreERT2 mice. Membranes were probed with primary antibodies to Cx26, Cx40, Cx45 and Cx43. Normalization to ß-actin levels revealed that the amount of Cx43 protein was reduced by 69±5% in tamoxifen-treated Cx43fl/fl:SM-CreERT2 animals, whereas the levels of other connexin proteins investigated remained unchanged (n=3).