Fig. 4. Inhibition of Rho effectors attenuates EF-induced growth cone guidance. Format is as in Fig. 1. (A) Mean angle turned during 5 hours. No drug + EF data are repeated from Fig. 1 for comparison; ns, not significant; ^#P<0.05; ^##P=0.0002; ^###P<0.0001. (B) Frequency of cathodal or anodal turning. ^*P<0.05; ^***P<0.001. (C) Rate of growth cone advance in an EF. ^##P=0.0002; ^###P<0.0001. (D) Composite drawings of neurons in an EF (cathode to the left) for 5 hours. Compare with no drug + EF in Fig. 1D. Bar, 100 µm for all drawings.