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Figure 2


Fig. 2. Actin and microtubule staining of growth cones exposed to an EF of 150 mV/mm (cathode to the left) in the presence of cytoskeleton inhibitors. Confocal images of Rhodamine-phalloidin (red) staining of F-actin and {alpha}-tubulin (green). (A) No drug + EF. (B,C,F) Taxol (tax) + EF. Arrow in C indicates looped microtubules in the central region of the growth cone, enlarged in the inset. Arrow in F indicates the swelling that sometimes forms in neurons exposed to >20 nM taxol. It marks the point on the neurite where the growth cone was at the time of drug addition. (D,E) Latrunculin A (lat) + EF. Inset in D shows detail of the growth cone in the white rectangle. Actin staining in 25 nM latrunculin is punctate, indicated by arrow in E. (F) Taxol + EF. (G) Vinblastine + EF, Bars, 10 µm.